|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Mapping of the nuclear matrix-bound chromatin hubs by a new M3C experimental procedureстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Gavrilov A.A., Zukher I.S., Philonenko E.S., Razin S.V., Iarovaia O.V.
- Журнал: Nucleic Acids Research
- Том: 38
- Номер: 22
- Год издания: 2010
- Издательство: Oxford University Press
- Местоположение издательства: United Kingdom
- Первая страница: 8051
- Последняя страница: 8060
- DOI: 10.1093/nar/gkq712
- Аннотация: We have developed an experimental procedure to analyze the spatial proximity of nuclear matrix-bound DNA fragments. This protocol, referred to as Matrix 3C (M3C), includes a high salt extraction of nuclei, the removal of distal parts of unfolded DNA loops using restriction enzyme treatment, ligation of the nuclear matrix-bound DNA fragments and a subsequent analysis of ligation frequencies. Using the M3C procedure, we have demonstrated that CpG islands of at least three housekeeping genes that surround the chicken α-globin gene domain are assembled into a complex (presumably, a transcription factory) that is stabilized by the nuclear matrix in both erythroid and non-erythroid cells. In erythroid cells, the regulatory elements of the α-globin genes are attracted to this complex to form a new assembly: an active chromatin hub that is linked to the pre-existing transcription factory. The erythroid-specific part of the assembly is removed by high salt extraction. Based on these observations, we propose that mixed transcription factories that mediate the transcription of both housekeeping and tissue-specific genes are composed of a permanent compartment containing integrated into the nuclear matrix promoters of housekeeping genes and a ’guest’ compartment where promoters and regulatory elements of tissue-specific genes can be temporarily recruited.
- Добавил в систему: Разин Сергей Владимирович