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Amphipathic CRAC-Containing Peptides Derived from the Influenza Virus A M1 Protein Modulate Cholesterol-Dependent Activity of Cultured IC-21 Macrophagesстатья Перевод
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 сентября 2018 г.
- Авторы: Dunina-Barkovskaya A.Ya, Vishnyakova Kh S., Golovko A.O., Arutyunyan A.M., Baratova L.A., Bathishchev O.V., Radyukhin V.A.
- Журнал: Biochemistry (Moscow)
- Том: 83
- Номер: 8
- Год издания: 2018
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 982
- Последняя страница: 991
- Оригинальные версии: АМФИПАТИЧЕСКИЕ CRAC-СОДЕРЖАЩИЕ ПЕПТИДЫ-ФРАГМЕНТЫ БЕЛКА М1 ВИРУСА ГРИППА МОДУЛИРУЮТ ХОЛЕСТЕРИН-ЗАВИСИМУЮ АКТИВНОСТЬ МАКРОФАГОВ IC-21
- DOI: 10.1134/S0006297918080096
- Аннотация: Entry of many viral and bacterial pathogens into host cells depends on cholesterol and/or cholesterol-enriched domains (lipid rafts) in the cell membrane. Earlier, we showed that influenza virus A matrix protein M1 contains amphipathic α-helices with exposed cholesterol-recognizing amino acid consensus (CRAC) motifs. In order to test possible functional activity of these motifs, we studied the effects of three synthetic peptides corresponding to the CRAC-containing α-helices of the viral M1 protein on the phagocytic activity of cultured mouse IC-21 macrophages. The following peptides were used: LEVLMEWLKTR (M1 α-helix 3, a.a. 39–49; further referred to as peptide 1), NNMDKAVKLYRKLK (M1 α-helix 6, a.a. 91–105; peptide 2), and GLKNDLLENLQAYQKR (M1 α-helix 13, a.a. 228–243; peptide 3). We found that all three peptides modulated interactions of IC-21 macrophages with non-opsonized 2-μm target particles. The greatest effect was demonstrated by peptide 2: in the presence of 35 μM peptide 2, the phagocytic index of IC-21 macrophages exceeded the control value by 60%; 10–11 mM methyl-β-cyclodextrin abolished this effect. Peptides 1 and 3 exerted weak inhibitory effect in a narrow concentration range of 5–10 μM. The dose-response curves could be approximated by a sum of two (stimulatory and inhibitory) components with different Hill coefficients, suggesting existence of at least two peptide-binding sites with different affinities on the cell surface. CD spectroscopy confirmed that the peptides exhibit structural flexibility in solutions. Altogether, our data indicate that amphipathic CRAC-containing peptides derived from the viral M1 protein modulate lipid raft-dependent processes in IC-21 macrophages.
- Добавил в систему: Батищев Олег Вячеславович