|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
A New and Efficient Method for Synthesis of 5′‐Conjugates of Oligonucleotides through Amide‐Bond Formation on Solid Phaseстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Anna V Kachalova, Dmitry A Stetsenko, Elena A Romanova, Vadim N Tashlitsky, Michael J Gait, Tatiana S Oretskaya
- Журнал: Helvetica Chimica Acta
- Том: 85
- Номер: 8
- Год издания: 2002
- Издательство: Verlag Helvetica Chimica Acta
- Местоположение издательства: Switzerland
- Первая страница: 2409
- Последняя страница: 2416
- Аннотация: An efficient method for synthesis of oligonucleotide 5′-conjugates through amide-bond formation on solid phase is described. Protected oligonucleotides containing a 5′-carboxylic acid function were obtained by use of a novel non-nucleosidic phosphoramidite building block, where the carboxylic acid moiety was protected by a 2-chlorotrityl group. The protecting group is stable to the phosphoramidite coupling conditions used in solid-phase oligonucleotide assembly, but is easily deprotected by mild acidic treatment. The protecting group may be removed also by ammonolysis. 5′-Carboxylate-modified oligonucleotides were efficiently conjugated on solid support under normal peptide-coupling conditions to various amines or to the N-termini of small peptides to yield products of high purity. The method is well-suited in principle for the synthesis of peptide-oligonucleotide conjugates containing an amide linkage between the 5′-end of an oligonucleotide and the N-terminus of a peptide.
- Добавил в систему: Ташлицкий Вадим Неронович