An express method of testing activity of a repair enzyme, uracil-DNA-glycosylaseстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:A rapid and effective method of testing of a repair enzyme, uracil-DNA-glycosylase, was proposed. As a substrate, a deoxyuridine-containing 5'-P-32-labeled deoxyoligonucleotide covalently attached to a polystyrene support (Tenta Gel S-NH2) was used. The ammonia cleavage of the apyrimidine site formed in the enzymic reaction followed by the transition of the labeled oligonucleotide fragment from the solid phase into solution allowed the detection of the enzymic activity.