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Neurexin I alpha is a major alpha-latratoxin receptor that cooperates in alpha-latrotoxin actionстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 2 октября 2018 г.
- Авторы: Geppert, (Geppert M., M); Khvotchev, (Khvotchev M., M); Krasnoperov, (Krasnoperov V., V); Goda, (Goda Y., Y); Missler, (Missler M., M); Hammer, (Hammer RE, RE); Ichtchenko, (Ichtchenko K., K); Petrenko, (Petrenko AG, AG); Sudhof, (Sudhof TC, TC)
- Журнал: Journal of Biological Chemistry
- Том: 273
- Номер: 3
- Год издания: 1998
- Издательство: American Society for Biochemistry and Molecular Biology Inc.
- Местоположение издательства: United States
- Первая страница: 1705
- Последняя страница: 1710
- DOI: 10.1074/jbc.273.3.1705
- Аннотация: alpha-Latrotoxin is a potent neurotoxin from black widow spider venom that binds to presynaptic receptors and causes massive neurotransmitter release. A surprising finding was the biochemical description of two distinct cell surface proteins that bind alpha-latrotoxin with nanomolar affinities; Neurexin I alpha binds alpha-latrotoxin in a Ca2+- dependent manner, and CIRL/latrophilin binds in a Ca2+-independent manner. We have now generated and analyzed mice that lack neurexin I alpha to test its importance in alpha-latrotoxin action, alpha-Latrotoxin binding to brain membranes from mutant mice was decreased by almost 50% compared with wild type membranes; the decrease was almost entirely due to a loss of Ca2+-dependent alpha-latrotoxin binding sites. In cultured hippocampal neurons, alpha-latrotoxin was still capable of activating neurotransmission in the absence of neurexin I alpha. Direct measurements of [H-3]glutamate release from synaptosomes, however, showed a major decrease in the amount of release triggered by alpha-latrotoxin in the presence of Ca2+. Thus neurexin I alpha is not essential for alpha-latrotoxin action but contributes to alpha-latrotoxin action when Ca2+ is present. Viewed as a whole, our results show that mice contain two distinct types of alpha-latrotoxin receptors with similar affinities and abundance but different properties and functions. The action of alpha-latrotoxin may therefore be mediated by independent parallel pathways, of which the CIRL/latrophilin pathway is sufficient for neurotransmitter release, whereas the neurexin I alpha pathway contributes to the Ca2+-dependent action of alpha-latrotoxin.
- Добавил в систему: Петренко Александр Георгиевич