|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Сalibration-free analysis of zooplankton by laser-induced breakdown spectroscopy (LIBS)тезисы доклада
- Авторы: Sushkov N., Lobus N., Zaytsev S., Popov A., Labutin T.
- Сборник: IX International conference Plasma Physics and Plasma Techology (PPPT-9), Contributed papers
- Тезисы
- Год издания: 2018
- Место издания: Kovcheg Minsk
- Первая страница: 372
- Последняя страница: 373
- Аннотация: It has recently been discovered that Kara Sea copepods accumulate trace elements like lithium, arsenic and uranium [1]. Moreover, determination of Mg/Ca, Sr/Ca and other element ratios in biota provides insight into environmental conditions [2]. Application of LIBS for the analysis of plankton samples is limited by the absence of suitable CRMs. Therefore, a calibration-free (CF) approach [3] is of a great interest for the solution of this problem. This study was focused on determination of ratios between Li, Na, K, Mg, Ca, Sr, and Al in zooplankton. Initially, we compared harmonics of a Nd-YAG laser (532 nm, 355 and 266 nm) for sampling of pelleted plankton specimens. The 2nd harmonic (532 nm) prevented reliable measurements with a sufficient signal-to-noise ratio due to the formation of big craters with a relatively low plasma emissivity, and the 3rd and 4th harmonics provided a much higher lines intensity in plasma spectra. We used a 0.5–4 μs time interval to ensure local thermodynamic equilibrium in nanosecond laser plasma following literature data. Temperature and electron density were calculated from CN molecular bands in the 383 nm region, hydrogen (Hα and Hβ) and magnesium (at 383 and 517 nm) atomic lines. We selected lines for CF calculation free from self-absorption and spectral interferences with the use of a synthetic spectrum (an approximation of light transport in a homogeneous plasma). Temporal evolution of the plasma was traced over 0.5–3 μs delays. Minimum level of disturbing factors was observed at early delays (0.5–0.75 μs), which is not surprising given generally low plasma temperature, low line intensity and reproducibility of spectra owing to the features of the object under investigation. Analytical measurements were performed with the smallest possible gate width to minimize the influence of plasma variations. Accuracy of the results was proved by common ICP-OES analysis. At the moment, the proposed method for the determination of element ratios in animal tissues is suitable for their semi-quantitative determination. Acknowledgements. The reported study was funded by RFBR according to the research project № 18-33-01297. References 1. Lobus N.V. Oceanology, 56(2016), 809–818. 2. Marali S. et al. Palaeogeography, Palaeoclimatology, Palaeoecology, 484(2017) 109–128. 3. Ciucci A. et al. Applied Spectroscopy, 53(1999), 960–964.
- Добавил в систему: Сушков Николай Иванович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | SushkovNI_et_al_PPPT9_2018_Proceedings_upUNjc0.pdf | SushkovNI_et_al_PPPT9_2018_Proceedings_upUNjc0.pdf | 1,4 МБ | 21 декабря 2018 [nikolaisushkov] | |
| 2. | Полный текст | SushkovNI_et_al_PPPT9_2018_Proceedings.pdf | 291,0 КБ | 21 декабря 2018 [nikolaisushkov] |