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Use of a pyrimidine-dependent mutant for effective labeling of the DNA OF Aspergillus nidulansстатья
Информация о цитировании статьи получена из
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 мая 2015 г.
- Авторы: Zinchenko V.V., Грошев В.В., Kameneva S.V.
- Журнал: Mikrobiologiia
- Том: 47
- Номер: 6
- Год издания: 1978
- Издательство: Izdatel'stva Nauka
- Местоположение издательства: Russian Federation
- Первая страница: 1044
- Последняя страница: 1048
- Аннотация: Incorporation of the radioactive label from pyrimidines into RNA and DNA of an Aspergillus nidulans pyrimidine-dependent mutant was studied. The label from [14C2]uridine was incorporated at the highest rate into both fractions of nucleic acids from A. nidulans of the wild type and pyr-mutant. The content of the label in the DNA of the pyr-mutant was several times higher than that in the wild type DNA. Therefore, pyr-mutants can be used in order to study molecular-genetic processes in the cells of A. nidulans. The label from [3H5]deoxyuridine, [14C2]thymine, [14C2]thymidine, [14C2]cytosine and I114C2]uracil was incorporated into the pyr-mutant RNA and DNA at a much lower rate than into the wild type RNA and DNA. Apparently, these pyrimidines cannot maintain the growth of the pyr-mutant. Incorporation of the label from [14C2]uracil into the pyr-mutant DNA increased if growth of the mycelium was stimulated by low concentrations of unlabeled uridine.
- Добавил в систему: Зинченко Владислав Владимирович