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Comparison of the 'chemical' and 'structural' approaches to the optimization of the thrombin-binding aptamerстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 22 декабря 2015 г.
- Авторы: Tatarinova Olga, Tsvetkov Vladimir, Basmanov Dmitry, Barinov Nikolay, Smirnov Igor, Timofeev Edward, Kaluzhny Dmitry, Chuvilin Andrey, Klinov Dmitry, Varizhuk Anna, Pozmogova Galina
- Журнал: PLoS ONE
- Том: 9
- Номер: 2
- Год издания: 2014
- Издательство: Public Library of Science
- Местоположение издательства: United States
- Первая страница: e89383
- DOI: 10.1371/journal.pone.0089383
- Аннотация: Noncanonically structured DNA aptamers to thrombin were examined. Two different approaches were used to improve stability, binding affinity and biological activity of a known thrombin-binding aptamer. These approaches are chemical modification and the addition of a duplex module to the aptamer core structure. Several chemically modified aptamers and the duplex-bearing ones were all studied under the same conditions by a set of widely known and some relatively new methods. A number of the thrombin-binding aptamer analogs have demonstrated improved characteristics. Most importantly, the study allowed us to compare directly the two approaches to aptamer optimization and to analyze their relative advantages and disadvantages as well as their potential in drug design and fundamental studies.
- Добавил в систему: Tsvetkov Vladimir Borisovich