Marginal blebbing during the early stages of TNF-induced apoptosis indicates alteration in actomyosin contractilityстатья
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Авторы:
Domnina L.V. ,
Ivanova O.Y. ,
Pletjushkina O.Y. ,
Fetisova E.K. ,
Chernyak B.V. ,
Skulachev V.P. ,
Vasiliev J.M.
Журнал:
Cell Biology International
Том:
28
Номер:
6
Год издания:
2004
Издательство:
Academic Press
Местоположение издательства:
United States
Первая страница:
471
Последняя страница:
475
DOI:
10.1016/j.cellbi.2004.03.017
Аннотация:
Dynamics of alterations of cell surface topography during TNF-induced apoptosis of HeLa cells was examined by phase-contrast videomicroscopy and immunomorphological analysis. The final stage of apoptosis accompanied by cell rounding and general blebbing of the cell surface became after 4-6h of incubation but much earlier, after 1.5-3h, essentially flattened lamellipodia at the active edges transformed into the small blebs that were continuously extended and retracted during the next 1-2h. This phenomenon was called "marginal blebbing". It took place before the cytochrome c release from mitochondria to cytosol. Marginal blebbing was inhibited by drugs that depolymerized actin microfilaments (cytochalasin, latrunculin) or decreased Rho-kinase-dependent contractility of actin-myosin cortex (H7, HA-1077, Y27632). A pancaspase inhibitor, zVAD-fmk, completely prevented marginal and general blebbing, and TNF-induced apoptosis. DEVD-fmk, a specific inhibitor of caspase-3, inhibited both marginal and general blebbing but not cell rounding and death. Thus, marginal blebbing is an early microfilament-dependent apoptotic event. It is suggested that it is initiated by minimal activation of caspase-3 and the following local Rho-kinase-dependent stimulation of actin-myosin cortex contractility. Localization of marginal blebs at the active edge may be associated with special organization of cortex in that zone. © 2004 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
Добавил в систему:
Домнина Лидия Владимировна