Methods for isolation and enumeration of fungi developing in marine sediments

Bubnova, E.N.; Georgieva, M.L.; Grum-Grzhimailo, O.A.

Авторы: Bubnova E.N., Georgieva M.L., Grum-Grzhimailo O.A.
Журнал: Microbiology
Том: 87
Номер: 6
Год издания: 2018
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 777
Последняя страница: 782
Оригинальные версии: Способ выделения и учёта грибов, развивающихся в морских грунтах
DOI: 10.1134/S0026261718060061
Аннотация: A new design for a small growth container which was used for investigation of the microbiota of the White Sea littoral and sublittoral sediments is presented. These container made it possible to observe development of fungal mycelium under conditions of a natural ecotope (marine sediments) and to isolate this mycelium as pure cultures. The microbiota isolated from the containers was compared to that obtained by standard plating method of the same sediments. The mycelium developed in 10% of the containers installed. Intensity of growth was found to below in the sublittoral than in the littoral. Most fungi isolated from the containers did not produce spores in pure cultures. Apart from non-sporulating cultures, colonies of marine species Paradendryphiella salina and Acremonium fuci were obtained. Standard plating of the sediments often resulted in isolation of Penicillium and Tolypocladium species, while the share of sterile isolates was considerably lower than in the case of isolation using growth containers.
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Methods for isolation and enumeration of fungi developing in marine sedimentsстатья

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