Optimization of fractionation and structural analysis of the reactivating factor of Luteococcus japonicus subsp. casei

Авторы: Rogozhin E.A., Vorob’eva L.I., Khodzhaev E.Yu, Gerasimov E.S.
Журнал: Microbiology
Том: 88
Номер: 2
Год издания: 2019
Издательство: Pleiades Publishing, Ltd
Местоположение издательства: Road Town, United Kingdom
Первая страница: 132
Последняя страница: 136
DOI: 10.1134/S0026261719020097
Аннотация: 15 polypeptide fractions from the culture liquid of Luteococcus casei were obtained by means of solid-phase extraction on a hydrophobic sorbent with a C8 phase, followed by evaporation, lyophilization and fractionation using RP-HPLC. Only two of them (Nos. I and IV) showed reactivating activity against cells subjected to the UV-stress. Using the mass spectral analysis, the structural characteristic was obtained for fraction IV, which includes two components: Ala-Pro-Asn-Glu-Asn-Gln-Gly and Ala-Pro-Asn-Glu-Gln-Gly. In the database of primary structures of polypeptides, no similarity was found to the known full-length functional peptide molecules. In the genome of L. casei, such sequences are also not identified. Presumably, the formation of active L. casei polypeptides is associated with non-matrix synthesis, possibly with proteolysis of a large protein.
Добавил в систему: Ходжаев Евгений Юрьевич

	ИСТИНА	Войти в систему Регистрация
	ИПМех РАН
	Главная Поиск Статистика О проекте Помощь

ИСТИНА