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Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. V. Study of single-stranded cleavagesстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Yolov A.A., Gromova E.S., Kubareva E.A., Potapov V.K., Shabarova Z.A.
- Журнал: Nucleic Acids Research
- Том: 13
- Номер: 24
- Год издания: 1985
- Издательство: Oxford University Press
- Местоположение издательства: United Kingdom
- Первая страница: 8969
- Последняя страница: 8981
- DOI: 10.1093/nar/13.24.8969
- Аннотация: Concatemer DNA duplexes which contain at the EcoRII restriction endonuclease cleavage sites phospho-diester, phosphoamide or pyrophosphate internucleotide bonds have been synthesized. It has been shown that this enzyme did not cleave the substrate at phosphoamide bond. EcoRII endonuclease catalyzes single-strand cleavages both in dA- and dT-containing strands of the recognition site if the cleavage of the other strand has been blocked by modification of scissile bond or if the other strand has been cleaved. This enzyme interacts with both strands of the DNA recognition site, each of them being cleaved independently on the cleavage of another one. Nucleotide sequences flanking the EcoRII site on both sides are necessary for effective cleavage of the substrate.
- Добавил в систему: Кубарева Елена Александровна