NF-kB p50 subunit cross-linking to DNA duplexes, containing a monosubstituted pyrophosphate internucleotide bondстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:The new express technique based on the use of BrCN to synthesize DNA duplexes, containing non-substituted or monosubstituted pyrophosphate internucleotide bonds has been proposed, Using this technique, DNA duplexes having modified internucleotide bonds between dT and dC residues in the human NF-kappa B transcription factor recognition sequence in HIV-1 (5'-GGAAAGTCCC-3') have been prepared. We demonstrate that these internucleotide bonds within the recognition site do not prevent the formation of NF-kappa B p50 subunit complex with the corresponding duplexes. The cross-linking of NF-kappa B p50 subunit to the DIVA duplex containing a monosubstituted pyrophosphate internucleotide bond has been successfully performed.