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Design of new reagents on the base of DNA duplexes for irreversible inhibition of transcription factor NF-kBстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Kozlov I.A., Kubareva E.A., Ivanovskaya M.G., Shabarova Z.A.
- Журнал: Antisense & nucleic acid drug development
- Том: 7
- Номер: 4
- Год издания: 1997
- Издательство: Mary Ann Liebert, Inc.
- Местоположение издательства: New Rochelle, NY, United States
- Первая страница: 279
- Последняя страница: 289
- DOI: 10.1089/oli.1.1997.7.279
- Аннотация: The main purpose of the present work is to search for the optimal design of a DNA duplex containing an active group for crosslinking and irreversible inhibition of the transcription factor NF-kappa B. Modified DNA duplexes with an identical nucleotide sequence but different internucleotide phosphates replaced by the trisubstituted pyrophosphate internucleotide group were synthesized. Crosslinking of the human NF-kappa B p50 subunit with the modified DNA duplexes was carried out. It was shown that only four modified duplexes crosslinked with the NF-kappa B p50 subunit. The specificity of these reactions was confirmed. A position of the phosphate in the NF-kappa B recognition site was found where replacement on the active trisubstituted pyrophosphate group resulted in a 50% yield of crosslinking. The fact that DNA duplexes containing the trisubstituted pyrophosphate group specifically react with the NF-kappa B p50 subunit in the Escherichia coli total lysate supports the idea that such modified DNA can be used as high specific inhibitors for DNA-recognizing proteins.
- Добавил в систему: Кубарева Елена Александровна