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Role of paracrine factors secreted by mesenchymal stromal cells in spermatogonial stem cell niche regulationтезисы доклада
Информация о цитировании статьи получена из
Web of Science
Дата последнего поиска статьи во внешних источниках: 21 мая 2019 г.
- Авторы: Sagaradze G.D., Basalova N.A., Kirpatovsky V.I., Ohobotov D.A., Grigorieva O.A., Kamalov A.A., Efimenko A.Yu
- Сборник: Human Gene Therapy
- Серия: A2–A169 (November 2018)
- Том: 27
- Тезисы
- Год издания: 2018
- Место издания: Mary Ann Liebert Inc United States United States
- Первая страница: 136
- Последняя страница: 136
- Аннотация: A stem cell niche is a structural and functional microenvi- ronment supporting and regulating a stem cell pool. Mesench- ymal stromal cells (MSC), as a part of stem cell niche, maintain many types of tissue-specific resident stem cells particularly through the production of multiple bioactive factors and thus coordinating processes of tissue repair and regeneration. In spermatogonial stem cell (SSC) niche, cells functionally similar to MSC were also found, but their role remained uncovered. However, MSC can be crucial for SSC niche regulation as they produce paracrine factors affecting principal components of SSC niche by modulating cell proliferation, survival and differentia- tion. We studied the contribution of MSC secretome to SSC niche regulation within the development of a new MSC secretome-based drug for the treatment of male infertility. Using a model of rat bilateral abdominal cryptorchidism for reversible SSC niche injury we observed that MSC secretome components effectively restored spermatogenesis. By immunohistochemical analysis of testis sections we observed regeneration of the whole testicle, not only distinct seminiferous tubules. A direct corre- lation between number of tubules containing many proliferating cells and produced spermatozoa number, and an inverse corre- lation between the interstitial cell proliferation level and pro- duced spermatozoa number were found. We also conducted in vitro evaluation of survival and proliferation of specific SSC niche cells to confirm in vivo data. Our findings suggest MSC could regulate SSC niche by paracrine signals and support spermatogenesis.
- Добавил в систему: Сагарадзе Георгий Дмитриевич