Recognition of Model Analyte Mixtures in the Presence of Blood Plasma Using a Mixture of Fluorophores (“Fluorescent Tongueстатья
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Дата последнего поиска статьи во внешних источниках: 6 февраля 2019 г.
Аннотация:The paper is devoted to the development of a version of the fingerprint method based on the effectof analytes on the shape of the fluorescence spectrum of a fluorophore mixture. The model analytes were medicinal substances (amikacin, sulfamethoxazole, pyracetam, and chloramphenicol) and binary to quater-nary mixtures of these substances in equal concentrations. The mixtures were recognized using a fluorophore whose fluorescence was quenched to different extent by different model analytes (CdSe/CdS/ZnS quantum dots, Schiff base prepared from o-phthalic dialdehyde and polyethyleneimine, and also rhodamine B and flu-orescein immobilized on silica nanoparticles to increase the degree of quenching – Rhod/SiO2 and Fluor/SiO2). The fluorophores were used as mixtures, “fluorescent reagents” containing from one to four fluorophores. The classes of analytes were distinguished by calculating Mahalanobis distances on score plots of the principal component method. It was found that it was more difficult to distinguish analyte mixtures in the presence of blood plasma than in a buffer solutionand that the best f luorescent reagent was the mixture of all four fluorophores, which ensured the subdivision of 15 mixtures of 2–4 model analytes into seven classes. An alternative fingerprint method based on the use of UV absorption spectra allowed distinguishing of only five classes.