|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Нокодазол приводит к инактивации центров организации микротрубочек и появлению областей локальной полимеризации микротрубочек в К-митозестатья
Дата последнего поиска статьи во внешних источниках: 22 ноября 2015 г.
- Авторы: Алиева И.Б., Воробьев И.А.
- Журнал: Биополимеры и клетка
- Том: 4
- Номер: 2
- Год издания: 1988
- Издательство: Наукова Думка
- Местоположение издательства: Киев
- Первая страница: 91
- Последняя страница: 100
- DOI: 10.7124/bc.000217
- Аннотация: Under the action of nocodasole at a concentration of 0.02 μg/ml the mitotic spindle is preserved in PE cells. Nocodazole at a concentration of 0.2 μg/ml results in the appearance of microtubule (MT) convergence centres lacking the centrioles and/or chromosomes. Bundles of MT bypass kinetochores, kinetochores become 1.5–3-times as long. At a nocodazole concentration of 0.02–0.2 μg/ml the daughter centriole becomes 1.5–2-fold as long. Under the action of nocodazole at a concentration of 0.6 μg/ml all chromosomes are packed in a slot-like structure. Kinetochores of several chromosomes surrounding one «slot» may be situated on their inner surfaces. Then short disorderly MT are observed in such a «slot». The centrioles are not surrounded by MT. In each cell one diplosome (rarely both) disintegrate. It is supposed that the appearance of three- and tetrapolar mitoses in tissue culture cells is due to the disintegration of one or both diplosomes, respectively.
- Добавил в систему: Алиева Ирина Борисовна