[The lipase/lipoxygenase bienzyme system in AOT reversed micelles in octane]

Авторы: Pavlenko I.M., Kuptsova O.S., Kliachko N.L., Levashov A.V.
Журнал: Bioorganicheskaia khimiia
Том: 28
Номер: 1
Год издания: 2002
Первая страница: 50
Последняя страница: 55
Аннотация: In this work it is shown that the bienzyme lipase/lipoxygenase system can function in reversed micelles of bis(2-ethyl)hexyl sulfosuccinate (AOT) in octane. As a lipase substrate, a fish fat preparation (fat of sea mammals) with a high content of polyunsaturated fatty acids was used. It was demonstrated that the bienzyme reaction proceeded in a stationary mode and had a rate-limiting step catalyzed by lipase. Under optimal conditions, the efficacy of functioning of the bienzyme system was by an order of magnitude higher than that in water. The lipase/lipoxygenase bienzyme system can be used as a new method of spectrophotometric determination of lipase activity. The English version of the paper.
Добавил в систему: Клячко Наталья Львовна

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