Mechano-chemical effects of Ca2+ in cross-linked troponin-C filmsстатья
Статья опубликована в высокорейтинговом журнале
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:Changes in troponin C (TnC) conformation upon Ca2+ binding forms the basis for regulatory and structural functions of TnC molecules. In the present study, Ca2+-induced conformational changes in TnC were observed by mechanical measurements. TnC films were prepared by drying or electrospraying TnC solutions, cross-linked with glutaraldehyde, and isometric tension and stiffness measured as a function of pCa. An increase in Ca2+ from a pCa of 9 to 4 induced large-scale mechanical changes in the TnC films causing several percent shrinkage of the unloaded films. This shrinkage could be partially assigned to Ca2+ binding to the Ca2+/Mg2+ sites of TnC. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.