|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Development of a surface plasmon resonance immunosensor and ELISA for 3-nitrotyrosine in human urineстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 21 февраля 2019 г.
- Авторы: Qiyi He, Chen Yingshan, Shen Ding, Cui Xiping, Zhang Chunguo, Yang Huiyi, Zhong Wenying, Eremin Sergei A., Fang Yanxiong, Zhao Suqing
- Журнал: Talanta
- Том: 195
- Год издания: 2019
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 655
- Последняя страница: 661
- DOI: 10.1016/j.talanta.2018.11.110
- Аннотация: 3-Nitrotyrosine (3-NT) is thought to be a relevant biomarker of nitrosative stress which is associated with many inflammatory and chronic diseases. It is necessary to develop confidential method for specific and sensitive 3-NT detection. In this paper, on the basis of anti-3-NT specific antibody, we developed a label-free indirect competitive surface plasmon resonance (SPR) immunosensor and ELISA for the detection of 3-NT. Under the optimized conditions, the SPR immunosensor can obtain a linear range of 0.17–6.07 μg/mL and a limit of detection (LOD) of 0.12 μg/mL while the ELISA can reach 0.33–9.94 μg/mL and a LOD of 0.24 μg/mL.The selectivity of 3- NT was also testified by six kinds of amino acid analogues. Besides, the developed SPR immunosensor was compared thoroughly with a conventional ELISA in spiked analysis of urine samples. Good recoveries and correlation between these two methods were observed (R2 =0.964). Therefore, it is concluded that the automated SPR platform can be applied to quantify 3-NT in biological samples with its sensitivity, accuracy, and realtiming
- Добавил в систему: Еремин Сергей Александрович
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|