Basic guidelines for the selection of polyelectrolytes that can effectively prevent thermal aggregation of enzymes without any substantial loss in their catalytic activityстатья
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Web of Science ,
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Авторы:
Stogov S.V.a ,
Muronets V.I.a,
Izumrudov V.A.b
Журнал:
Polymer Science - Series C
Том:
53
Номер:
1
Год издания:
2011
Издательство:
Maik Nauka/Interperiodica Publishing
Местоположение издательства:
Russian Federation
Первая страница:
97
Последняя страница:
106
DOI:
10.1134/S1811238211030027
Аннотация:
Factors governing thermal aggregation and thermal denaturation of a model homotetrametric enzyme, glyceraldehyde-3-phosphate dehydrogenase, electrostatically bound to a polyelectrolyte are revealed. Polyanions with sulfate or sulfonate groups in chains are the most efficient suppressors of aggregation. However, their presence promotes thermal denaturation. Short chains of relatively hydrophobic polyanions exert a strong denaturing effect that changes the secondary, tertiary, and quaternary structures of a protein. Basic guidelines for the selection of a polyelectrolyte are outlined. According to the proposed guidelines, hydrophilic high-molecular-mass macromolecules able to form stable soluble polyelectrolyte complexes with the enzyme are the most efficient at preventing thermal aggregation without substantial loss in catalytic activity. © Pleiades Publishing, Ltd., 2011.
Добавил в систему:
Изумрудов Владимир Алексеевич