Direct capture of plasmid DNA from non-clarified bacterial lysate using polycation-grafted monolithsстатья
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Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Авторы:
Hanora A.a.b ,
Savina I.a. ,
Plieva F.M.c ,
Izumrudov V.A.d ,
Mattiasson B.a.c ,
Galaev Yu I.a.c
Журнал:
Journal of Biotechnology
Том:
123
Номер:
3
Год издания:
2006
Издательство:
Elsevier BV
Местоположение издательства:
Netherlands
Первая страница:
343
Последняя страница:
355
DOI:
10.1016/j.jbiotec.2005.11.017
Аннотация:
Monolith columns from macroporous polyacrylamide gel were grafted with polycations, poly(N,N-dimethylaminoethyl methacrylate) (polyDMAEMA), (2-(methacryloyloxy)ethyl)-trimethyl ammonium chloride (polyMETA) and partially quaternized polyDMAEMA prepared via treating polyDMAEMA-grafted columns with propylbromide. The polymer grafting degrees varied between 34 and 110%. The polycation-grafted monolithic columns are able to capture plasmid DNA directly from alkaline lysate of Escherichia coli cells. Due to the large pore size in macroporous monoliths the particulate material present in non-clarified feeds did not block the columns. The captured plasmid DNA was eluted with 1 M NaCl as particulate-free preparation with significantly reduced content of protein and RNA as compared to the applied lysate. © 2005 Elsevier B.V. All rights reserved.
Добавил в систему:
Изумрудов Владимир Алексеевич