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Enhanced rapidity for qualitative detection of Listerial monocytogenes using an enzyme-linked lmmunosorbent assay and immuniochromatography strip test combined with immunomagnetic bead separationстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Shim Won-Bo, Choi Jin-Gil, Kim Ji-Young, Yang Zheng-You, Lee Kyu-Ho, Kim Min-Gon, Sang-Do Ha, Kim Keun-Sung, Kim Kwang-Yup, Kim Cheol-Ho, Eremin Sergei A., Chung Duck-Hwa
- Журнал: Journal of Food Protection
- Том: 71
- Номер: 4
- Год издания: 2008
- Издательство: International Association for Food Protection
- Местоположение издательства: United States
- Первая страница: 781
- Последняя страница: 789
- Аннотация: An enzyme-linked immunosorbent assay (ELISA), immunochromatography (ICG) strip test, and immunomagnetic bead separation (IMBS) system based on a monoclonal antibody were individually developed for the detection and isolation of Listeria monocytogenes in meat samples. The three methods showed a strong reaction with Listeria species and a weak reaction with Staphylococcus aureus. To increase the rapidity of L. monocytogenes detection, combinations of the ELISA and ICG strip test with the IMBS system (ELISA-IMBS and ICG-IMBS) were investigated. In comparative analyses of artificially inoculated meat and samples of processed meat, the ELISA and ICG strip test required 24 h of enrichment time to detect the inoculated meat samples with >= 1 X 10(2) CFU/10 g, whereas the ELISA-IMBS and ICG-IMBS required only 14 h of enrichment. Analyses of naturally contaminated meat samples (30 pork samples, 20 beef samples, 26 chicken samples, 20 fish samples, and 20 processed meat samples) performed by ELISA-IMBS, ICG-IMBS, and API kit produced similar results. The ELISA-IMBS and ICG-IMBS provide a more rapid assay than the individual ELISA and the ICG strip test and are appropriate for rapid and qualitative detection of L. monocytogenes (or Listeria species) in meat samples. With the ICG-IMBS, L. monocytogenes could be detected in meat samples within 15 h and the method has potential as a rapid, cost-effective on-site screening tool for the detection of L. monocytogenes in food samples and agricultural products at a minimum detection level of similar to 100 CFU/10 g.
- Добавил в систему: Еремин Сергей Александрович