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Interactions of glyceraldehyde-3-phosphate dehydrogenase with G- and F-actin predicted by Brownian dynamicsстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Ouporov I.V., Knull HR, Lowe SL, Thomasson KA
- Журнал: Journal of Molecular Recognition
- Том: 14
- Номер: 1
- Год издания: 2001
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 29
- DOI: 10.1002/1099-1352(200101/02)14:1<29::AID-JMR517>3.0.CO;2-T
- Аннотация: Brownian dynamics (BD) was used to simulate the binding of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) to G- and F-actin, High-resolution three-dimensional models (X-ray and homology built) of the proteins were used in the simulations. The electrostatic potential about each protein was predicted by solving the linearized Poisson-Boltzmann equation for use in ED simulations. The ED simulations resulted in complexes of GAPDH with G- or F-actin involving positively charged surface patches on GAPDH (Lyses 24, 69, 110 and 114) and negatively charged residues of the N- and C-termini (Asps 1, 25 and 363 and Glus 2, 4, 224 and 364) of actin, The actin residues all belong to subdomain 1, Although the positively charged surface patches of GAPDH are not close enough to each other to enhance their electrostatic potential, occasionally two subunits of the GAPDH tetramer may simultaneously interact with two neighboring monomers of F-actin, These results are different from those of fructose-1,6-bisphosphate aldolase, where quaternary structure directly influenced binding by two subunits combining their electrostatic potentials (see previous study, Ouporov ed al,, 1999, Biophys, J, 76: 17-27), Instead, GAPDH uses its quaternary structure to span the distance between two different actin subunits so that it can interact with two different actin subunits simultaneously. Copyright (C) 2000 John Wiley & Sons, Ltd.
- Добавил в систему: Упоров Игорь Владимирович