Aluminum-tolerant Pseudomonas fluorescens: ROS toxicity and enhanced NADPH productionстатья
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Авторы:
Singh R.a. ,
Beriault R.a. ,
Middaugh J.a. ,
Hamel R.b. ,
Chenier D.a. ,
Appanna V.D.a,
Kalyuzhnyi S.c.
Журнал:
Extremophiles
Том:
9
Номер:
5
Год издания:
2005
Издательство:
Springer Verlag
Местоположение издательства:
Germany
Первая страница:
367
Последняя страница:
373
DOI:
10.1007/s00792-005-0450-7
Аннотация:
Aluminum (Al) triggered a marked increase in reactive oxygen species (ROS) such as O 2- and H2O2 in Pseudomonas fluorescens. Although the Al-stressed cells were characterized with higher amounts of oxidized lipids and proteins than controls, NADPH production was markedly increased in these cells. Blue native polyacrylamide gel electrophoresis (BN-PAGE) analyses coupled with activity and Coomassie staining revealed that NADP+ -dependent isocitrate dehydrogenase (ICDH, E.C. 1.1.1.42) and glucose-6-phosphate dehydrogenase (G6PDH, E.C. 1.1.1.49) played a pivotal role in diminishing the oxidative environment promoted by Al. These enzymes were overexpressed in the Al-tolerant microbes and were modulated by the presence of either Al or hydrogen peroxide (H2O2) or menadione. The activity of superoxide dismutase (SOD, E.C. 1.15.1.1), an enzyme known to combat ROS stress was also increased in the cells cultured in millimolar amounts of Al. Hence, Al-tolerant P. fluorescens invokes an anti-oxidative defense strategy in order to survive. © Springer-Verlag 2005.
Добавил в систему:
Калюжный Сергей Владимирович