|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Phosphorylase kinase phosphorylation of skeletal-muscle troponin Tстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Risnik V.V., Dobrovolskii A.B., Gusev N.B., Severin S.E.
- Журнал: Biochemical Journal
- Том: 191
- Номер: 3
- Год издания: 1980
- Издательство: Portland Press, Ltd.
- Местоположение издательства: United Kingdom
- Первая страница: 851
- Последняя страница: 854
- Аннотация: Rabbit skeletal-muscle troponin T was phosphorylated by a standard preparation of phosphorylase kinase [Cohen (1973) Eur. J. Biochem. 34, 1–14] and by fractions obtained after chromatography of phosphorylase kinase on phosphocellulose. The original preparation of phosphorylase kinase phosphorylated at least two sites, one of which was serine-1. The second and probably the third sites were presumably located in the peptide flanked by amino-acid residues 147 and 161 of troponin T. Fractions of phosphorylase kinase was adsorbed on phosphocellulose phosphorylated only the second site. Tightly adsorbed fractions possessed high troponin T kinase and phosvitin kinase activities and phosphorylated only serine-1 of troponin T. The results suggest that standard preparations of phosphorylase kinase are contaminated by troponin T kinase, which can phosphorylate serine-1 of troponin T.
- Добавил в систему: Гусев Николай Борисович