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Formation of an Efficient Energy Transfer Complex between Quantum Dots and Exiguobacterium sibiricum Retinal Protein via the Histidine–Cysteine Anchorстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 апреля 2019 г.
- Авторы: Lukashev E.P., Petrovskaya L.E., Tretyak M.V., Kryukova E.A., Sizova S.V., Oleinikov V.A.
- Журнал: Russian Journal of Bioorganic Chemistry
- Том: 44
- Номер: 6
- Год издания: 2018
- Издательство: Maik Nauka/Interperiodica Publishing
- Местоположение издательства: Russian Federation
- Первая страница: 687
- Последняя страница: 694
- DOI: 10.1134/S1068162018050114
- Аннотация: Resonance energy transfer in self-assembled hybrid structures formed by water-soluble semiconductor CdSe/ZnS quantum dots (QD) with a cysteine shell and the Exiguobacterium sibiricum retinal protein (ESR) in three modifications, with and without a 6-membered C-terminal histidine tag, and with both the histidine tag and C-terminal cysteine residue. Steady-state and time-resolved fluorescence spectroscopy was used to demonstrate that nonradiative energy transfer from QD onto the ESR protein depends on the strength of intermolecular interactions in the hybrid complex. A shift from electrostatic interactions in the QD–ESR donor–acceptor pair to coordinate bond with an additional disulfide bond resulted in an increase of the energy transfer efficiency from 40 to over 90%. The proposed method to produce noncovalent conjugates from QDs and light-sensitive proteins can be a promising one for biomedical applications, as well as for the development of new solar energy accumulation systems.
- Добавил в систему: Лукашев Евгений Павлович