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A novel type of energetics in a marine alkali-tolerant bacterium: Δμ-Na-driven motility and sodium cycleстатья
Информация о цитировании статьи получена из
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Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Chernyak B.V., Dibrov P.A., Glagolev A.N., Sherman M.Yu, Skulachev V.P.
- Журнал: FEBS Letters
- Том: 164
- Номер: 1
- Год издания: 1983
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 38
- Последняя страница: 42
- DOI: 10.1016/0014-5793(83)80014-3
- Аннотация: Motility of a marine alkali-tolerant bacterium, Vibrio alginolyticus, can be observed in the presence of high concentrations of a protonophorous uncoupler, CCCP. Motility in the CCCP-containing media is completely inhibited by decrease in extracellular [Na+] or by monensin-induced increase in intracellular [Na+]. A mutant has been selected that grows only in media supplemented with a substrate such as acetate requiring no Δμ-Na to be transported into the cell. Motility of the mutant was found to be completely inhibited by CCCP. Cyanide, CCCP and vanadate added separately or in twos inhibit motility only partially. The three poisons added together completely paralyse the cells. In this inhibitor cocktail, arsenate can substitute for CCCP + vanadate; cyanide can be replaced by anaerobiosis. It is concluded that (i) Δμ-Na rather than Δμ-NH powers the flagellar motor of V. alginolyticus in the presence of CCCP, and (ii) in addition to the Na+-motive respiratory chain [Tokuda, H. and Unemoto, T. (1982) J. Biol. Chem. 257, 10007–10014] there is a vanadate and arsenate-sensitive oxygen-independent mechanism of ΔμNa generation, presumably an ion-motive ATPase. A suggestion is put forward that circulation of Na+ can replace that of H+ in V. alginolyticus, Δμ-Na being formed by the Na+-motive respiratory chain and utilized by Na+-solute symporters, the Na+-driven flagellar motor and maybe by a reverse ion-motive ATPase.
- Добавил в систему: Черняк Борис Викторович