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The effects of prenatal hypoxia at the beginning of organogenesis on neuronal resistance to oxidative stressстатья
Информация о цитировании статьи получена из
Web of Science
Дата последнего поиска статьи во внешних источниках: 29 мая 2015 г.
- Авторы: Graf A.V., Maslova M.V., Buligina E.R., Sokolova N.A.
- Сборник: European Neuropsychopharmacology
- Серия: Papers of the 19th ECNP Congress
- Том: 16
- Год издания: 2006
- Издательство: Elsevier BV
- Местоположение издательства: Netherlands
- Первая страница: 251
- DOI: 10.1016/S0924-977X(06)70220-6
- Аннотация: The reason of different diseases of newborns and prepuberty often lies in prenatal life, where the lack of oxygen is considered to be one of the main interfering factors. Hypoxy-ishemic damage of brain tissue in prenatal period is considered to be one of the main causes of newborn’s death and isuspected to increase the risk of schizophrenia, epilepsy, deficit hyperactivity disorder, mental. The females of nonlinear white rats were subjected to acute hypobaric hypoxia on the 9−10th day of gestation corresponding to the beginning of organogenesis. Using flow cytometry technique we examined the neuronal resistance to oxidative stress induced by preincubation of cells with H2O2. The primary culture of neurons was isolated from cerebellums of 8−10-day-old rats. Tissue slices was processed by collagenaza with following perturbation and filtering cells through teflon strainer. Experimental group consisted of rat pups born from females, subjected to acute hypoxia on the 9−10th day of gestation. The determination of active forms of oxygen was performed using the method of flow cytometry with the help of fluorescent probe – H2DCFDA (2’,7’-dichlorodihydrofluorescein diacetate). The amount of dead cells in suspension was determined from the fluorescence of propidium iodide, glutathione content – from fluorescence of CMFDA (5-chloromethylfluorescein diacetate). Active forms of oxygen content in neurons of control and experimental animals did not significantly differ. Preincubation of neuron suspension with 10mM H2O2 for 30 min led to the significant increase of active forms of oxygen level either in experimental or in control group, though H2O2-induced enhance in the latter case was 1,5−6 times less. The increased level of active forms of oxygen was not accompanied with elevation in amount of dead cells. Preincubation of cell suspension with 3 mM CMFDA has shown that glutathione content in neurons, isolated from brain of experimental animals, was 1,3−2 times lower than in control cells. Thereby, oxidative stress-induced increase of active forms of oxygen level in animals survived from prenatal hypoxia correlates with baseline low level of glutathione content. As glutathione seems to be one of the main cellular antioxidants, the decrease of its level suggests greater sensitivity of experimental animals to oxidative stress.
- Добавил в систему: Граф Анастасия Викторовна