Polyelectrolyte complexes as a tool for purification of plasmid DNA: Background and developmentстатья
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Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Авторы:
Wahlund P.O a ,
Gustavsson P.E b ,
Izumrudov V.A.c,
Larsson P.O b ,
Galaev I.Yu a
Журнал:
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Том:
807
Номер:
1
Год издания:
2004
Издательство:
Elsevier BV
Местоположение издательства:
Netherlands
Первая страница:
121
Последняя страница:
127
DOI:
10.1016/j.jchromb.2004.01.046
Аннотация:
The demand for highly purified plasmids in gene therapy and plasmid-based vaccines requires large-scale production of pharmaceutical-grade plasmid. Plasmid DNA was selectively precipitated from a clarified alkaline lysate using the polycation poly(N,N′-dimethyldiallylammonium) chloride which formed insoluble polyelectrolyte complex (PEC) with the plasmid DNA. Soluble PECs of DNA with polycations have earlier been used for cell transformation, but now the focus has been on insoluble PECs. Both DNA and RNA form stable PECs with synthetic polycations. However, it was possible to find a range of salt concentration where plasmid DNA was quantitatively precipitated whereas RNA remained in solution. The precipitated plasmid DNA was resolubilised at high salt concentration and the polycation was removed by gel-filtration. © 2004 Elsevier B.V. All rights reserved.
Добавил в систему:
Изумрудов Владимир Алексеевич