|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Identification of a Region of the Polypeptide Chain of Na,KATPase α-Subunit Interacting with 67kDa Melittin Like Proteinстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 26 августа 2016 г.
- Авторы: Kamanina Yu V., Klimanova E.A., Dergousova E.A., Petrushanko I.Yu, Lopina O.D.
- Журнал: Biochemistry (Moscow)
- Том: 81
- Номер: 3
- Год издания: 2016
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 369
- Последняя страница: 375
- DOI: 10.1134/S000629791603007X
- Аннотация: It was shown earlier that a 67kDa protein purified from mouse kidney using polyclonal antibodies against melittin (a peptide from bee venom) interacted with Na,KATPase from rabbit kidney. In this study, a 43kDa proteolytic fragment of Na,KATPase αsubunit interacting with the 67kDa melittinlike protein was found. The αsubunit washydrolyzed by trypsin in the presence of 0.5 mM ouabain (E2conformation of Na,KATPase). A proteolytic fragmentinteracting with the 67kDa melittinlike protein that was identified by massspectrometry is a region of the cytoplasmicdomain of Na,KATPase αsubunit located between amino acid residues 591 and 775. The fragment includes a conservative DPPRA motif that occurs in many Ptype ATPases. It was shown earlier that this motif of H,KATPase from gastricmucosa binds to melittin. We suggest that namely this motif of Ptype ATPases is able to interact with proteins containingmelittinlike modules.DOI: 10.1134/S000629
- Добавил в систему: Климанова Елизавета Андреевна
Прикрепленные файлы
| № | Имя | Описание | Имя файла | Размер | Добавлен |
|---|---|---|---|---|---|
| 1. | Полный текст | Identification_of_a_Region_of_the_Polypeptide_Chain_of_NaKA… | 838,5 КБ | 5 апреля 2016 [KlimanovaEA] |