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eIF4G2 balances its own mRNA translation via a PCBP2-based feedback loopстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 15 сентября 2020 г.
- Авторы: Smirnova Victoria V., Shestakova Ekaterina D., Bikmetov Dmitry V., Chugunova Anastasiya A., Osterman Ilya A., Serebryakova Marina V., Sergeeva Olga V., Zatsepin Timofei S., Shatsky Ivan N., Terenin Ilya M.
- Журнал: RNA
- Том: 25
- Номер: 7
- Год издания: 2019
- Первая страница: 757
- Последняя страница: 767
- DOI: 10.1261/rna.065623.118
- Аннотация: Poly(rC)-binding protein 2 (PCBP2, hnRNP E2) is one of the most abundant RNA-binding proteins in mammalian cells. In humans, it exists in seven isoforms, which are assumed to play similar roles in cells. The protein is shown to bind 3’ untranslated regions (3’ UTRs) of many mRNAs and regulate their translation and/or stability, but nothing is known about functional consequences of PCBP2 binding to 5’ UTRs. Here we show that the PCBP2 isoform f interacts with the 5’ UTRs of mRNAs encoding eIF4G2 (a translation initiation factor with yet unknown mechanism of action, also known as DAP5) and Cyclin I, and inhibits their translation in vitro and in cultured cells, while the PCBP2 isoform e only affects Cyclin I translation. Furthermore, eIF4G2 participates in a cap-dependent translation of the PCBP2 mRNA. Thus, PCBP2 and eIF4G2 seem to regulate one another’s expression via a novel type of feedback loop formed by the translation initiation factor and the RNA-binding protein.
- Добавил в систему: Теренин Илья Михайлович