Molecular origin of surface-enhanced Raman spectra of E. coli suspensions excited at 532 and 785 nm using silver nanoparticle sols as SERS substrates

Durovich, E.A.; Evtushenko, E.G.; Senko, O.V.; Stepanov, N.A.; Efremenko, E.N.; Eremenko, A.V.; Kurochkin, I.N.

Авторы: Durovich E.A., Evtushenko E.G., Senko O.V., Stepanov N.A., Efremenko E.N., Eremenko A.V., Kurochkin I.N.
Журнал: Bulletin of RSMU
Том: 7
Номер: 6
Год издания: 2018
Издательство: Pirogov Russian National Research Medical University
Местоположение издательства: Moscow
Первая страница: 25
Последняя страница: 32
DOI: 10.24075/brsmu.2018.088
Аннотация: Research into the molecular origin of surface-enhanced Raman spectra (SERS) of bacteria is a crucial step in assessing the future of SERS-based discrimination and identification of bacteria in clinical analysis, food quality control, etc. Previous studies have revealed that at 785 nm excitation wavelength SERS of bacterial cells placed on a solid surface functionalized with in-situ grown aggregated gold nanoparticles covered with SiO 2 originate from a mixture of 6 purine derivatives (adenine, guanine, AMP, hypoxanthine, xanthine, and uric acid) that are released by the cells into the medium. The aim of the present work was to investigate whether such interpretation is possible with a different class of SERS substrates: silver nanoparticle sols at excitation wavelengths of 785 and 532 nm. The suspension of the Escherichia coli DH5α strain was used as a model bacterium. Sols of silver nanoparticles were obtained by reducing silver nitrate in the presence of alkaline hydroxylamine hydrochloride. Number-weighted mean hydrodynamic diameter of the particles was 43±2 nm. We confirm that at both excitation wavelengths the spectra can be best described as a superposition of 4 purine derivatives: adenine, guanine, hypoxanthine, and xanthine. Importantly, we have discovered that 1) the spectra of the purine mixture are characteristic of viable cells only; 2) due to the variations in the concentrations of purine metabolites released by the cells into the surrounding medium the spectra of a bacterial strain can vary significantly when a silver nanoparticle sol is used as a SERS substrate.
Добавил в систему: Сенько Ольга Витальевна

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Molecular origin of surface-enhanced Raman spectra of E. coli suspensions excited at 532 and 785 nm using silver nanoparticle sols as SERS substratesстатья

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