|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
DNA barcodes effectively identify the morphologically similar Common Opossum (Didelphis marsupialis) and Virginia Opossum (Didelphis virginiana) from areas of sympatry in Mexicoстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 6 апреля 2016 г.
- Авторы: Cervantes Fernando A., Arcangeli Jesica, Hortelano-Moncada Yolanda, Borisenko Alex V.
- Журнал: Mitochondrial DNA
- Том: 21
- Год издания: 2010
- Издательство: Informa Healthcare
- Местоположение издательства: United Kingdom
- Первая страница: 44
- Последняя страница: 50
- DOI: 10.3109/19401736.2010.538051
- Аннотация: Two morphologically similar species of opossum from the genus Didelphis--Didelphis virginiana and Didelphis marsupialis--cooccur sympatrically in Mexico. High intraspecific variation complicates their morphological discrimination, under both field and museum conditions. This study aims to evaluate the utility and reliability of using DNA barcodes (short standardized genome fragments used for DNA-based identification) to distinguish these two species. Sequences of the cytochrome c oxidase subunit I (Cox1) mitochondrial gene were obtained from 12 D. marsupialis and 29 D. virginiana individuals and were compared using the neighbor-joining (NJ) algorithm with Kimura's two-parameter (K2P) model of nucleotide substitution. Average K2P distances were 1.56% within D. virginiana and 1.65% in D. marsupialis. Interspecific distances between D. virginiana and D. marsupialis varied from 7.8 to 9.3% and their barcode sequences formed distinct non-overlapping clusters on NJ trees. All sympatric specimens of both species were effectively discriminated, confirming the utility of Cox1 barcoding as a tool for taxonomic identification of these morphologically similar taxa.</.
- Добавил в систему: Борисенко Алексей Владимирович