|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Preparation of myristoylated and nonmyristoylated recombinant recoverins in E-coli and comparison of their functional activitiesстатья
Информация о цитировании статьи получена из
Scopus,
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 25 июля 2019 г.
- Авторы: Zargarov A.A., Senin I.I., Alekseev A.M., ShulgaMorskoi S.V., Philippov P.P., Lipkin V.M.
- Журнал: Bioorganicheskaya Khimiya
- Том: 22
- Номер: 7
- Год издания: 1996
- Издательство: Izdatel'stva Nauka
- Местоположение издательства: Russian Federation
- Первая страница: 483
- Последняя страница: 488
- Аннотация: A recombinant plasmid was constructed for expressing a gene for bovine recoverin under the control of the lac promoter. Coexpression of the recoverin and N-myristoyl transferase genes was performed to prepare recombinant myristoylated recoverin. The obtained systems provide high levels of biosynthesis of the recombinant recoverins in the E. coli cells. Using a reconstructed system, containing urea washed rod outer segment membranes, purified rhodopsin kinase (RK), and a recoverin, it was shown that the three recoverin forms (natural, recombinant nonmyristoylated, and recombinant myristoylated ones) perform the calcium-dependent regulation of the activity of RK with half a maximum effect at a free calcium concentration of 2 mu M. Interestingly, the N-terminal myristoylation of recoverin increased substantially its functional activity.
- Добавил в систему: Филиппов Павел Павлович