Stabilization of the enzyme–substrate complex of the mutant Asp-67Asn inorganic pyrophosphatase from Escherichia coli by fluoride ionsстатья
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Дата последнего поиска статьи во внешних источниках: 7 октября 2013 г.
Местоположение издательства:Road Town, United Kingdom
Первая страница:169
Последняя страница:174
Аннотация:Magnesium-supported PPi hydrolysis by the mutant Asp-67Asn E. coli pyrophosphatase at saturating PPi and metal-activator concentrations in the presence of NaF is followed by a gradual decrease in the initial rate of PPi hydrolysis. The reaction occurs in two steps: first a complex containing enzyme, pyrophosphate, magnesium, and fluoride ions is immediately formed, then its conformation changes slowly. This enzyme--substrate complex stabilized by fluoride is partially active and can be isolated by the removal of excess fluoride by gel-filtration.