Аннотация:A method of RNA extraction with ammonium trichloroacetate (ATCA), the low toxic chaotropic agent, and the filter paper (cotton
wool) for the RNA purification from ATCA-propanol-2 solution was developed. The yield and purity of total RNA isolated from
plant and animal viruses, E.coli, from animal cells and plants were identical to those obtained by the conventional methods using
toxic and hazardous agents such as guanidine thiocyanate, phenol, chloroform, etc. The quality of RNA was evaluated by the
comparison of their optical and electrophoretic characteristics with those obtained after SDS-phenol deproteinization. Mass
molecular diagnostics of virus and viroid infections was performed using home-made hand juice squeezer with changeable plastic
shells and a vacuum manifold with mini column nests compatible with the immune plate. Use of low toxic ATCA is of particular
importance for quality RNA isolation from all kinds of tissues and mass molecular diagnosis of the infecting pathogens
(epiphytotic, epizootics, pandemics).