|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Defect of vacuolar protein sorting stimulates proteolytic processing of human urokinase-type plasminogen activator in the yeast Hansenula polymorphaстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
- Авторы: Agaphonov M., Romanova N., Sokolov S., Iline A., Kalebina T., Gellissen G., Ter-Avanesyan M.
- Журнал: FEMS Yeast Research
- Том: 5
- Номер: 11
- Год издания: 2005
- Издательство: Blackwell
- Местоположение издательства: United Kingdom
- Первая страница: 1029
- Последняя страница: 1035
- Аннотация: Human urokinase-type plasminogen activator (uPA) is poorly secreted by yeast cells. Here, we have selected Hansenula polymorpha mutants with increased productivity of active extracellular uPA. Several of the obtained mutants also demonstrated a defect of sorting of carboxypeptidase Y to the vacuole and the mutant loci have been identified in six of them. All these mutations damaged genes involved in protein traffic between the Golgi apparatus and the vacuole, namely PEP3, VPS8, VPS10, VPS17, and VPS35. We have shown that inactivation of the VPS10 gene encoding the vacuolar protein sorting receptor does not increase uPA secretion but stimulates its proteolytic processing.
- Добавил в систему: Соколов Святослав Сергеевич