Biosensitive element in the form of immobilized luminescent photobacteria for detecting ecotoxicants in aqueous flow‐through systems

Efremenko, E.N.; Maslova, O.V.; Kholstov, A.V.; Senko, O.V.; Ismailov, A.D.

Авторы: Efremenko E.N., Maslova O.V., Kholstov A.V., Senko O.V., Ismailov A.D.
Журнал: Luminescence : the journal of biological and chemical luminescence
Том: 31
Номер: 6
Год издания: 2016
Издательство: John Wiley & Sons Inc.
Местоположение издательства: United States
Первая страница: 1283
Последняя страница: 1289
DOI: 10.1002/bio.3104
Аннотация: We demonstrated the possibility of long-term and efficient application of a biosensitive element (BE) in the form of Photobacterium phosphoreum photobacteria immobilized in poly(vinyl alcohol) (PVA) cryogel for detecting various ecotoxicants (Zn2+, Cu2+, Hg2+, Pb2+, 2,4-dichlorophenoxyacetic acid, 2,6-dimethylphenol, pentachlorophenol, coumaphos, malathion, chlorpyrifos and methyl parathion) in flow-through media. The range of detectable concentrations of ecotoxicants was determined at 1 × 10−8 to 1 × 10−4 M for heavy metal ions and at 1 × 10−8 to 1 × 10−5 M for phenol derivatives and organophosphorus pesticides. Immobilized cells of photobacteria quantitatively reacted with these ecotoxicants; cell sensitivity exhibited no flow rate dependence in the range from 45 to 180 mL/h. At a constant concentration of ecotoxicant in the flow, the bioluminescence quenching profile of immobilized cells demonstrated an integral response. The BE could remain in a flow-through medium for at least 10 days while retaining 95% of luminescent activity in the absence of ecotoxicants. The BE tested in this work was demonstrated to have a long shelf life (> 60 weeks) at −80°C without changes in the baseline level of bioluminescence.
Добавил в систему: Маслова Ольга Васильевна

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Biosensitive element in the form of immobilized luminescent photobacteria for detecting ecotoxicants in aqueous flow‐through systemsстатья

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