|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Increased production of reactive oxygen species hypertrophied myocardium: in vivo study by microdialysis techniqueстатья Тезисы
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 мая 2015 г.
- Авторы: Ruuge E., Gorodetskaya E.A., Kalenikova E.I., Murashev A.N., Medvedev O.S.
- Журнал: European Heart Journal
- Том: 23
- Номер: Supplement: S
- Год издания: 2002
- Издательство: Oxford University Press
- Местоположение издательства: United Kingdom
- Первая страница: 671
- Последняя страница: 671
- Аннотация: The generation of hydroxyl radical (ОН•) in the myocardium of Wistar, Wistar-Kyoto (WKY) rats and SHR-SP using microdialysis technique was evaluated. Experiments were carried out on anaesthetized open-chest male rats (300-400 g) mechanically ventillated with room air supplement with oxygen. The linear microdialysis probe (molecular weight cut off 5,000) was inserted in the left ventrical (LV) wall at a depth of about 1.5 mm. The probe was perfused with salicylate-contaning Ringer solution at a rates 3 mkl/min. Dialysate sampling started 60 min after the probe implantation. 2,3-Dihydroxybenzoic acid (2,3-DHBA) produced in vivo in the reaction of ОН• with salicylate was used as a marker of ОН• generation and was measured by HPLC with electrochemical detection. When dialysate 2,3-DHBA reached a a baseline, 3-5 samples were collected for basal ОН• level estimation. Because of artifactual exogenous salicylic acid oxydation, some quantity of 2,3-DHBA was always presented in perfusion fluid. This value was assaid for earch animal and declared as 100% for following calculations. Mean baseline values of 2,3-DHBA in dialysate were not different (112+8.7%, n=5) from that in perfusion fluid in WKY rats, but were significantly lower (87+3.2% n= 6, p<0.03) in Wistar rats and significantly higher (154+1.1%, n=7, p<0.0001) in SHR-SP. Increased basal ОН• level in SHR-SP was positively correlated (r=0.831, n=7, p<0.05) with degree of LV hypertrophy expressed as a ratio of LV weight to the body weight.
- Добавил в систему: Городецкая Евгения Ароновна