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Application of femtosecond laser microsurgery in assisted reproductive technologies for preimplantation embryo taggingстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 10 июля 2019 г.
- Авторы: Ilina I.V., Khramova Y.V., Filatov M.A., Sitnikov D.S.
- Журнал: Biomedical optics express
- Том: 10
- Номер: 6
- Год издания: 2019
- Издательство: Optical Society of America
- Местоположение издательства: United States
- Первая страница: 2985
- Последняя страница: 2995
- DOI: 10.1364/BOE.10.002985
- Аннотация: Femtosecond laser pulses were applied for precise alphanumeric code engraving on the zona pellucida (ZP) of mouse zygotes for individual embryo marking and their identification. The optimal range of laser pulse energies required for safe ZP microsurgery has been determined. ZP was marked with codes in three different planes to simplify the process of embryo identification. No decrease in developmental rates and no morphological changes of embryos post laser-assisted engraving have been observed. ZP thickness of embryos post laser-assisted code engraving has been shown to differ significantly from that of control group embryos at the hatching stage. Due to moderate ZP thinning as compared to its initial width at 0.5 dpc (days post coitum), readability of the code degrades slightly and it still remains recognizable even at hatching stage. Our results demonstrate that application of femtosecond laser radiation could be an effective approach for noninvasive direct embryo tagging, enabling embryo identification for the whole period of preimplantation development.
- Добавил в систему: Храмова Юлия Владимировна