Surface characterization of the thermal remodeling helical plant virusстатьяИсследовательская статья
Статья опубликована в высокорейтинговом журнале
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Дата последнего поиска статьи во внешних источниках: 10 июля 2019 г.
Аннотация:Previously, we have reported that spherical particles (SPs) are formed by the thermalremodeling of rigid helical virions of native tobacco mosaic virus (TMV) at 94˚C. SPs haveremarkable features: stability, unique adsorption properties and immunostimulation poten-tial. Here we performed a comparative study of the amino acid composition of the SPs andvirions surface to characterize their properties and take an important step to understandingthe structure of SPs. The results of tritium planigraphy showed that thermal transformationof TMV leads to a significant increase in tritium label incorporation into the following sites ofSPs protein: 41–71 .a. and 93–122 a.a. At the same time, there was a decrease in tritiumlabel incorporation into the N- and C- terminal region (1–15 a.a., 142–158 a.a). The use ofcomplementary physico-chemical methods allowed us to carry out a detailed structural anal-ysis of the surface and to determine the most likely surface areas of SPs. The obtained datamake it possible to consider viral protein thermal rearrangements, and to open new opportu-nities for biologically active complex design using information about SPs surface amino acidcomposition and methods of non-specific adsorption and bioconjugation.