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KINETICS OF EXPRESSION OF LUCIFERASE GENES FROM THE FIREFLIES PHOTINUS-PYRALIS AND LUCIOLA-MINGRELICA IN ESCHERICHIA-COLI .2. ROLE OF PH IN THE BIOSYNTHESIS OF PROTEINS AND THEIR TRANSFORMATION INTO ACTIVE CONFORMERSстатья
Информация о цитировании статьи получена из
Web of Science
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 27 мая 2015 г.
- Авторы: LEONTEVA OV, KUTUZOVA GD, TUROVETSKII VB, UGAROVA NN
- Журнал: Biochemistry (Moscow)
- Том: 59
- Номер: 1
- Год издания: 1994
- Издательство: Pleiades Publishing, Ltd
- Местоположение издательства: Road Town, United Kingdom
- Первая страница: 85
- Последняя страница: 88
- Аннотация: The kinetics of expression of firefly luciferase genes from Photinus pyralis and Luciola mingrelica was studied in E. coli CA cells transformed with plasmids pME61 and pJG lambda, respectively, carrying the thermally inducible lambda P-R promoter, under conditions of shifted pH which increased the enzymatic activity. The maximal levels of luciferase from Ph. pyralis and L. mingrelica indicated by electrophoresis in 12% polyacrylamide gels in the presence of sodium dodecyl sulfate was 5.3 and 4.9%, respectively, of the total cell protein with the external pH shifted from 7.5 to 9.0; the corresponding levels were 4.7 and 4.5% with the internal pH shifted to 8.5. There was a correlation between enzyme activation and changes in external or internal pH.
- Добавил в систему: Туровецкий Валерий Борисович