Trace enrichment of phenylcarboxylic acids from a model biological fluid and serum of human blood

Popov, A.Y.; Blinnikova, Z.K.; Tsyurupa, M.P.; Davankov, V.A.

Авторы: Popov Alexander Y., Blinnikova Zinaida K., Tsyurupa Maria P., Davankov Vadim A.
Журнал: Journal of Separation Science
Том: 42
Номер: 16
Год издания: 2019
Издательство: John Wiley & Sons Ltd.
Местоположение издательства: United Kingdom
Первая страница: 2706
Последняя страница: 2714
DOI: 10.1002/jssc.201900258
Аннотация: The study was focused on the development of solid‐phase extraction protocol for seven phenylcarboxylic acids from albumin solutions by using unmodified hypercrosslinked polystyrene restricted access materials with crosslinking degrees varying from 100% to 400%. (Four of the acids are known to be markers of sepsis). The breakthrough volume of the most hydrophilic 3,4‐dihydroxybenzoic acid rises as the sorbent bridging extent grows. Inversely, the breakthrough volume of the most hydrophobic 3‐phenylpropionic acid was found to decrease considerably when the degree of crosslinking exceeds 200%. This unusual pattern is conditioned upon the superposition of two opposite tendencies. Increasing substitution extent of phenyls facilitates their π‐π‐interactions with polar compounds whereas rising density of the network reduces the accessibility of sorption sites to all solutes. Mini‐cartridges containing 30 mg of an optimal sorbent take up the acids completely and reversibly, the recoveries being close to 100% even in the presence of high concentrations of albumin. By coupling the developed solid‐phase extraction with HPLC‐DAD technique one managed to determine quantitatively phenylcarboxylic acids in serum of a healthy patient blood, at that the recoveries varied from 93 to 100% while LOQ amounted to (4 – 9)×10−7M.
Добавил в систему: Попов Александр Юриевич

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Trace enrichment of phenylcarboxylic acids from a model biological fluid and serum of human bloodстатья Исследовательская статья

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