Mapping the fine specificity of ABO monoclonal reagents with A and B type-specific function-spacer-lipid constructs in kodecytes and inkjet printed on paperстатья
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Дата последнего поиска статьи во внешних источниках: 17 мая 2016 г.
Аннотация:Monoclonal (MoAb) reagents are routinely
used and are usually very reliable for the serologic
determination of ABO blood types. However, the
fine specificity and cross-reactivity of these reagents
are often unknown, particularly against synthetic antigens
used in some diagnostic assays. If nonserologic
assays or very sensitive techniques other than those
specifically prescribed by the manufacturer are used,
then there is a risk of incorrect interpretation of results.
Forty-seven MoAbs
and two polyclonal ABO reagents were tested against
red blood cell (RBC) kodecytes prepared with A trisaccharide,
A Type 1, A Type 2, A Type 3, A Type 4, B trisaccharide,
B Type 1, B Type 2, acquired B
trisaccharide, and Lea trisaccharide function-spacer-lipid
(FSL) constructs. Natural RBCs were tested in parallel.
In addition these FSL constructs were printed onto
paper with a desktop inkjet printer and used in a novel
immunoassay that identifies reactivity through the
appearance of alphanumeric characters.
Mapping of MoAbs with kodecytes and
printed FSL constructs revealed a series of broad recognition
patterns. All ABO MoAbs tested were reactive
with the RBC dominant Type 2 ABO antigens. Unexpectedly
some anti-A reagents were reactive against
the B Type 1 antigen, while others were poorly reactive
with trisaccharide antigens.
All ABO MoAbs detect the RBC dominant
Type 2 ABO antigens; however, some reagents
may show minor reactivity with inappropriate blood
group antigens, which needs to be considered when
using these reagents in alternative or highly sensitive
analytic systems.