Peptide synthesis in dimethylformamide-based organic media catalysed by non-covalent chymotrypsin-polyelectrolyte complexesстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 18 июля 2013 г.
Аннотация:Chymotrypsin-catalysed kinetically-controlled peptide synthesis was studied in mixtures of dimethylformamide (DMF) and acetonitrile containing 6% water. The free enzyme showed no detectable activity with 60% or more DMF. However, by pre-forming a complex with polyacrylate (optimum 50 carboxyl groups to 1 enzyme molecule), activity and stability were good at 60% DMF, and reasonable at 70% DMF. In 60% DMF. the peptide Ac-Tyr-Lys-NH? could be made in 95% yield using this catalyst.