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Facioscapulohumeral dystrophy myoblasts efficiently repair moderate levels of oxidative DNA damageстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 28 июня 2016 г.
- Авторы: Bou Saada Yara, Dib Carla, Dmitriev Petr, Hamade Aline, Carnac Gilles, Laoudj-Chenivesse Dalila, Lipinski Marc, Vassetzky Yegor S.
- Журнал: Histochemistry and Cell Biology
- Том: 145
- Номер: 4
- Год издания: 2016
- Издательство: Springer Verlag
- Местоположение издательства: Germany
- Первая страница: 475
- Последняя страница: 483
- DOI: 10.1007/s00418-016-1410-2
- Аннотация: Facioscapulohumeral dystrophy (FSHD) is a progressive muscular dystrophy linked to a deletion of a subset of D4Z4 macrosatellite repeats accompanied by a chromatin relaxation of the D4Z4 array on chromosome 4q. In vitro, FSHD primary myoblasts show altered expression of oxidative-related genes and are more susceptible to oxidative stress. Double homeobox 4 (DUX4) gene, encoded within each D4Z4 unit, is normally transcriptionally silenced but is found aberrantly expressed in skeletal muscles of FSHD patients. Its expression leads to a deregulation of DUX4 target genes including those implicated in redox balance. Here, we assessed DNA repair efficiency of oxidative DNA damage in FSHD myoblasts and DUX4-transfected myoblasts. We have shown that the DNA repair activity is altered neither in FSHD myoblasts nor in immortalized human myoblasts transiently expressing DUX4. DNA damage caused by moderate doses of an oxidant is efficiently repaired while FSHD myoblasts exposed for 24 h to high levels of oxidative stress accumulated more DNA damage than normal myoblasts, suggesting that FSHD myoblasts remain more vulnerable to oxidative stress at high doses of oxidants.
- Добавил в систему: Васецкий Егор Сергеевич