|
ИСТИНА |
Войти в систему Регистрация |
ИПМех РАН |
||
Differences in transcription patterns between induced pluripotent stem cells produced from the same germ layer are erased upon differentiationстатья
Статья опубликована в высокорейтинговом журнале
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 24 мая 2016 г.
- Авторы: Pirozhkova I., Barat A., Dmitriev P., Kim E., Robert T., Guegan J., Laoudj-Chenivesse D., Carnac G., Bilhou-Nabera C., Busato F., Tost J., Lipinski M., VASSETZKY Y.S.
- Журнал: PLoS ONE
- Том: 8
- Год издания: 2013
- Издательство: Public Library of Science
- Местоположение издательства: United States
- Первая страница: 53033
- DOI: 10.1371/journal.pone.0053033
- Аннотация: Little is known about differences between induced pluripotent stem cells produced from tissues originating from the same germ layer. We have generated human myoblast-derived iPS cells by retroviral transduction of human primary myoblasts with the OCT3/4, SOX2, KLF4 and MYC coding sequences and compared them to iPS produced from human primary fibroblasts. When cultivated in vitro, these iPS cells proved similar to human embryonic stem cells in terms of morphology, expression of embryonic stemness markers and gene promoter methylation patterns. Embryonic bodies were derived that expressed endodermal, mesodermal as well as ectodermal markers. A comparative analysis of transcription patterns revealed significant differences in the gene expression pattern between myoblast- and fibroblast-derived iPS cells. However, these differences were reduced in the mesenchymal stem cells derived from the two iPS cell types were compared.
- Добавил в систему: Васецкий Егор Сергеевич