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T-antigen interactions with chromatin and p53 during the cell cycle in extracts from Xenopus eggsстатья
Информация о цитировании статьи получена из
Web of Science,
Scopus
Статья опубликована в журнале из списка Web of Science и/или Scopus
Дата последнего поиска статьи во внешних источниках: 24 мая 2016 г.
- Авторы: VASSETZKY Y.S., Tchang F., Fanning E., Méchali M.
- Журнал: Journal of Cellular Biochemistry
- Том: 75
- Год издания: 1999
- Издательство: John Wiley & Sons Inc.
- Местоположение издательства: United States
- Первая страница: 288
- Последняя страница: 299
- DOI: 10.1002/(SICI)1097-4644(19991101)75:2<288::AID-JCB11>3.0.CO;2-7
- Аннотация: The role of SV40 large tumor T-antigen in replication of viral DNA is well established, but it is still unclear how T-antigen triggers cellular replication and cell transformation in non-permissive cells. Here, we used Xenopus egg extracts which reproduce most nuclear events linked to the cell cycle in vitro to analyze its interaction with genomic chromatin during the cell cycle. We show that T-antigen associates with chromatin before the nuclear membrane formation, and further demonstrate that the nuclear membrane is not necessary for its import into the nucleus. We show that the interaction of T-antigen with the endogenous chromatin does not occur at replication foci nor at RPA pre-replication centers. Immunoprecipitations as well as sucrose gradient experiments, indicate that the endogenous pool of p53 interacts with T-antigen. In addition, a transient association of both proteins with the nuclear matrix is observed during the ongoing DNA synthesis. These data are discussed in view of the T-antigen and p53 activity during the cell cycle.
- Добавил в систему: Васецкий Егор Сергеевич