Efficient synthesis of DNA dumbbells using template-induced chemical ligation in double-stranded polynucleotides closed by minihairpin fragments

Kuznetsova, S.; Merenkova, I.; Kanevsky, I.

Авторы: Kuznetsova SA, Merenkova IN, Kanevsky IE
Журнал: Antisense & nucleic acid drug development
Том: 9
Номер: 1
Год издания: 1999
Издательство: Mary Ann Liebert, Inc.
Местоположение издательства: New Rochelle, NY, United States
Первая страница: 95
Последняя страница: 100
DOI: 10.1089/oli.1.1999.9.95
Аннотация: The chemical ligation of 17 50–54-membered nicked DNA dumbbells with different closing fragments, nick positions, and nucleotides facing the nick were investigated. T4, T5, GTA4C, GCGA2GC, and GCGA3GC sequences were chosen as the closing fragments. The nicks were placed in the center of the duplex stem or were adjacent to the closing fragments. N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide and cyanogen bromide were used as the condensing agents. We showed that the ligation efficiency is 10%–90% depending on the sequence of the closing fragments, nick position, and nucleotides facing the nick. Coupling yields of 80%–90% were observed when the nick was situated in the middle of the molecule between two T residues or was adjacent to GCGA2GC or GCGA3GC minihairpins. In the last case, the reacting 3′-phosphate and 5′-hydroxy groups were brought close together by only two base pair minihairpins. The coupling yields did not depend on the nature of the condensing agent. On the basis of the results obtained, we believe a rational design of nicked DNA dumbbells has been developed for efficient chemical synthesis of closed dumbbells.
Добавил в систему: Кузнецова Светлана Александровна

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Efficient synthesis of DNA dumbbells using template-induced chemical ligation in double-stranded polynucleotides closed by minihairpin fragmentsстатья

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